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Help....yeast transformations

McLaren, Robert James mclarer at kahu.lincoln.ac.nz
Tue Jul 26 19:05:43 EST 1994


  I've never done lithium acetate transformations before (I use spheroplast 
transformation), but it looks to me like your using far too much DNA.  With 
spheroplast transformation I acheived optimal transformation efficiency by 
transforming 200 microlitres (1.6 times 10 to the 7 cells) with 10 ng DNA.

  Robert McLaren

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