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to: dave horvath

Kathy Shire shirek at FHS.CSU.MCMASTER.CA
Tue Jul 26 17:23:06 EST 1994


Hi Dave!

Transformation of yeast by spheroplasting can be a real pain in the butt. 
I have an easy  Lithium Acetate\Sorbitol protocol that gives between 5x10 4 to
5x10 5 colonies per ug DNA.  If you would like to try this method please
let me know. (give me your fax number)

Kath






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