Yeast Club Members-
I'm a newcomer to the yeast scene. The novelty of the bread smell has
worn off a while ago, but I'm still an amateur. I have tried something
that works pretty well and saves me a lot of time. Someone else might get
some use out of it.
Freezing Competent Yeast Cells:
Small Scale Transformation Using LiAc (Current Protocols in Molecular Biology)
After the 1hr incubation at 30C in 500uL 0.1M LiAc, freeze cells (as is)
at -70C in 100uL aliquots. Thaw cells when needed at RT, add plasmid
+hsDNA and continue normally.
I get about 500colonies/ug. The frequency is low but if you want a
plasmid in your yeast in a hurry, it works every time!