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gene disruption with linear DNA

amitchell at mbcl.rutgers.edu amitchell at mbcl.rutgers.edu
Tue Jul 19 10:16:44 EST 1994

I am trying to disrupt a gene in Saccharomyces Cer. I am using the Kleckner cas
sette flanked by greater than 1Kb of a yeast gene. The protocol is the one-step
gene disruption using linear DNA  by Rothstein. As of yet, no luck using Li acetate, or
electroporation. Suggestions would be greatly appreciated. D. Toke

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