IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

gene disruption with linear DNA

amitchell at mbcl.rutgers.edu amitchell at mbcl.rutgers.edu
Tue Jul 19 10:16:44 EST 1994


I am trying to disrupt a gene in Saccharomyces Cer. I am using the Kleckner cas
sette flanked by greater than 1Kb of a yeast gene. The protocol is the one-step
gene disruption using linear DNA  by Rothstein. As of yet, no luck using Li acetate, or
electroporation. Suggestions would be greatly appreciated. D. Toke
   



More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net