I am studying calmodulin in S. pombe and I am planning to look at
salt sensitivity of various calmodulin mutants that I have generated. It
seems calmodulin is required for Ca++ sensitivity and Na+ tolerance in S.
cerevisiae. I plan to look at Na+ (or Li+), Ca++ and Mn++ sensitivity of
my pombe strains. My question is: Does anybody out there know what
concentrations of Ca++ or Mn++ affect fission yeast growth? Paul Young
did some good work on Na+ and Li+ tolerant mutants of pombe, so I have
something to go on there, but I am at a loss as to what amounts of
divalent cations to try. I would greatly appreciate any suggestions or
references that all you people out there might be able to suggest.
Anything you can add would be much appreciated. Best answer gets a free
sample of my latest homebrew. (Of course you have to come to the GSA meeting
in Seattle to collect!) Cheers!
Department of Biochemistry SJ-70
University of Washington
Seattle, WA 98195
moser at u.washington.edu