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sap3? (ade3)

Michael Moser moser at U.WASHINGTON.EDU
Thu Jul 14 18:29:29 EST 1994

Dear Buddies,
	Lenore writes:
>When I put this strain (Which is ade3, his7) through a cross
>Histidine auxotrophy segregated mostly 3:1, indicating that in addition to
>the his7 allele another mutation affecting His-ness was segregating -
>could this be sap3? 

	I just wanted to let everybody know that didn't already know: 

	ade3 mutants are phenotypically His- !

	The reason for this is somewhat unclear although it may be that
precursors of THF or tetrahydrofolate (the product of Ade3p or THF synthase)
may somehow be poisoning enzyme(s) in the histidine biosynthetic pathway. 
THF itself is not required for His biosynthesis although a related
compound tetrahydrobiopterin is. 
	The reason that His- was segregating strangely for Lenore was 
that ade3 was also segregating along with his7.  As to sap3 I have never 
heard of it.  Good luck!

Mike Moser
Department of Biochemistry  SJ-70
University of Washington
Seattle, WA  98195
Tel: 206-543-5354
FAX: 206-685-1792
moser at u.washington.edu

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