I received a strain harboring a sap3 mutation. Does anyone know what this
is and what the phenotype is? The strain has other markers that I want to use,
but I need to be sure that the sap3 mutation won't interfere with my screen. I
got this strain from a friend who got it from a friend, etc., so, alas, we
don't know its source. The other markers are: ade2 ade3 leu2 trp1 his7
can1 (no allele numbers indicated). I just want the ade2 and ade3 alleles.
When I put this strain through a cross Histidine auxotrophy segregated mostly
3:1, indicating that in addition to the his7 allele another mutation affecting
His-ness was segregating - could this be sap3?
Thanks for any help