Hi Charlie Hoffman et al. what is PKA in schizo pombe? Pyruvate kinase?
If so, recall that PK in S. cerev. was one of the set of CDC mutants
that was thought to be boring, because sure, you can't cycle without
energy, and so what. A similar boredom would apply to the schizopombe
germination mutant. However, maybe it's not boring, really, but what IS
Also, re: glucose receptor stuff, here's an old finding that I think has
never been explained: Sue Lillie and John Pringle did some studies of
glycogen metabolism in cerev. in which it was found that, when the
concentration of glucose was varied in a batch culture, it seemed that
glycogen accumulation started when the conc of glucose in the medium had
been halved (by consumption), and was independent of the culture density.
It was as if a sensor mechanism was responding to the derivative of the
glucose conc. vs time relationship, rather than the conc. alone. Sorry
I don't have the reference readily available, search Lillie and Pringle
on medline around mid 1980's. True, it's not necessarily receptor-related.
I lost track of the hexokinase II story. Can anyone summarize that?
rapr at med.pitt.edu