IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Problems with 5FOA

Achim Wach wach at urz.unibas.ch
Fri Jul 8 00:32:36 EST 1994

Hello to everybody out there!

I am a graduate student at the Biocenter in Basel and I have some problems
with using the 5-fluoroorotic acid (5-FOA) counter-selection in S.cerevisiae.
SD plates containing 5-FOA (1 mg/ml) have a low pH, i.e. about pH 4.5.
Is this the source of my problems and is it therefore necessary to buffer
5-FOA medium prior to use?
Can someone out there help me and perhaps point me to some relevant references?

Thanks, Horst Pick

Please send your replies to Wach at Urz.UniBas.CH

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net