RE: yeast colony PCR.
There was a long discussion on this group a while back. You might try
searching the yeast section of the stanford gopher server to find it.
We found that preboiling was death, and got our best results when we used
fresh colonies (2 days old), put them straight in the tube with all the
goodies, put the tube in the machine and turned it on. This was with
lichten at helix.nih.gov