here again a question concerning the 2-hybrid system:
I'm screening for interacting proteins using the - well-known - 2-hybrid
system. Clones with interacting proteins should show the phenotype His+
AND beta-Gal+ .
Screening on selective plates (SC-HIS/-LEU/-TRP), I get several hundred
His+ clones, and a few of them are even beta-Gal+.
I expected perhaps a few His+/plate (not several hundred). Why do I get
so many ?
Did anybody make the same experience or does anybody have an explanation
for this ?
Thanx in advance,
| ... usual disclaimer applies. |
| Dr. B. Popping |
| University of Durham |
| Dept. Biol. Sci. PHONE +44 91 374 2430 |
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| Durham DH1 3LE E-MAIL Bert.Popping at durham.ac.uk |
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