Dear Yeast netters,
Does anyone have a good protocol for doing immunoprecipitation with
35S-labelled yeast lysates, monoclonal supernatants and protein A
sepharose. I need some idea of the quantity of lysate I should use per
precipitation as well as anything on reducing non-specific binding to the
Univ. of Guelph,
Guelph, Ontario, Canada.
email: PMASTRAN at uoguelph.ca