dan at cubmol.bio.columbia.edu (Daniel Zabetakis) writes:
> Does anyone have experience in making dissecting needles from optical
>fiber? We've been doing it for a while, and when you get a good needle it
>works fine. But it is very difficult to get a good needle. The tip of the
>fiber almost always shatters when cut with a razor blade.
> Does anyone have a favourite technique that gives a better yield?
In our lab we find that using a couple of glass cover slips
instead of a razor blade gives cleaner edges. It still takes a number of
tries to get a good one, but not as many. You sort of "tiddly-wink" the
fiber, being careful to not break the coverslips, which, as you might
imagine, are pretty fragile.