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immunofluor.-photobleaching

bram at mbcf.stjude.org bram at mbcf.stjude.org
Tue Aug 30 16:35:48 EST 1994


In article <33igkk$1bf at news.umbc.edu>, bolognes at umbc.edu (Ms. Cynthia Bolognese; (BIOL; GRAD)) writes:
> First, I'd like to thank everyone for the advice on getting my yeast cells to 
> stick to the slide. I've had some success. The problem now is bleaching. My
> cells stain, I can see them in the periphery of the field of view, but as 
> soon as I bring them into the center for a good view, they VERY RAPIDLY 
> bleach. My secondary ab is DTAF conjugated (same excitation & emission 
> wavelengths as FITC). Is this bleaching a result of unstable secondary? my 
> staining technique? or the UV source? Any references or info would be greatly 
> appreciated.  
> 
> Thanks in advance!

Are you using a bleaching-inhibitor, such as Vectastain (from Vector
Laboratories)? 
 (Not affiliated in anyway with Vector, just a reasonably satisfied
 customer.)

rick



> 
> --
> ___________________________________________________________________________
> Cindy Bolognese		 |						  |
> e-mail address:  	 |    Qapla'!  (Klingon for Success!)             |
> bolognes at umbc7.umbc.edu	 |						  |
> ---------------------------------------------------------------------------
> 



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