In article <33igkk$1bf at news.umbc.edu>, bolognes at umbc.edu (Ms. Cynthia Bolognese; (BIOL; GRAD)) writes:
> First, I'd like to thank everyone for the advice on getting my yeast cells to
> stick to the slide. I've had some success. The problem now is bleaching. My
> cells stain, I can see them in the periphery of the field of view, but as
> soon as I bring them into the center for a good view, they VERY RAPIDLY
> bleach. My secondary ab is DTAF conjugated (same excitation & emission
> wavelengths as FITC). Is this bleaching a result of unstable secondary? my
> staining technique? or the UV source? Any references or info would be greatly
> appreciated.
>> Thanks in advance!
Are you using a bleaching-inhibitor, such as Vectastain (from Vector
Laboratories)?
(Not affiliated in anyway with Vector, just a reasonably satisfied
customer.)
rick
>> --
> ___________________________________________________________________________
> Cindy Bolognese | |
> e-mail address: | Qapla'! (Klingon for Success!) |
>bolognes at umbc7.umbc.edu | |
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>
