Sikorski and Hieter (Genetics 122: 19-27, 1989) describe
the construction of a bunch of vectors and budding yeast strains
for transformation. To knock out the wild-type genes in their
starting strains they made some transplacement vectors carrying
URA3 and deleted copies of trp1 and his3 (plasmids YRp14/trp1-
delta1 and YRp14/his3-delta200, respectively, in the paper).
Does anyone out there have these plasmids, or know where I
can get them?
b7jm at musicb.mcgill.ca