We suspect that the fusion proteins we have created for use in the yeast
2-hybrid system are being degraded when expressed in the strains
used for screening libraries. Is anyone familiar with a protease
deficient yeast strain that would be compatible with either Stan Fields'
2-hybrid assay or Roger Brent's interaction trap protocol? Strain
designations and/or contact names would be greatly appreciated.
Please respond directly to: mrclean at u.washington.edu
Thanks in advance.
Department of Microbiology
University of Washington
Mail Stop SC-42
Seattle, WA 98195