First, I'd like to thank everyone for the advice on getting my yeast cells to
stick to the slide. I've had some success. The problem now is bleaching. My
cells stain, I can see them in the periphery of the field of view, but as
soon as I bring them into the center for a good view, they VERY RAPIDLY
bleach. My secondary ab is DTAF conjugated (same excitation & emission
wavelengths as FITC). Is this bleaching a result of unstable secondary? my
staining technique? or the UV source? Any references or info would be greatly
appreciated.
Thanks in advance!
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___________________________________________________________________________
Cindy Bolognese | |
e-mail address: | Qapla'! (Klingon for Success!) |
bolognes at umbc7.umbc.edu | |
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