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overcooked plates etc.

Bert Popping Bert.Popping at durham.ac.uk
Thu Aug 25 05:18:44 EST 1994

: Dear DanZ (good to hear from you again!) et al.,

: of course you are right that not every autoclaving with sugar results
: in dark plates, but it happens often enough to be cautious. And what
: concerns the taste of the yeasties, this is not just anecdotes. I got
: recently a strain on a dark (burnt sienna brown) plate from our neighbors
: at the University who routinelly autoclave everything together. They
: claimed that the yeast is sick and it indeed looked so; however, the
: culture almost miraculously recovered on a light, non-caramelized
: plate. So, it obviously does matter...

: Fatima

Dear Fatima,

surprisingly enough, most of our S.cerevisiae strains prefer obviously the caramelized 
sugar. The strains grow to higher densities in a liquid dark-brown YPDA medium.
These strains grow on light-brown YPDA plates as well as on dark brown YPDA plates.
But - of course - this might depend on the specific strain.  

| 	Dr. B. Popping				                          | 
| 	University of Durham			    			  |
| 	Dept. Biol. Sci. 	  PHONE   +44 91 374 2430 	          |
| 	South Road                FAX     +44 91 374 2417 	          |
| 	Durham DH1 3LE	          E-MAIL  Bert.Popping at durham.ac.uk       |
| 	ENGLAND			  					  |
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|	WWW set pointer to:   http://www.dur.ac.uk/~dbl0zz3 (Dept. Biol.) |

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