IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

soft or mushy or black plates

Dan Zabetakis dan at cubsps.bio.columbia.edu
Wed Aug 24 09:20:37 EST 1994

In article <33err9$20m at mserv1.dl.ac.uk>,  <FATIMA at AIMP.UNA.AC.AT> wrote:

>is indeed hydrolysis of the agar. It makes a difference when you autoclave
>takes too long to get to the right temperature - (sorry: I meant YOUR auto-
>clave but I cannot return to correct it).

   Get a real mailer :-)

> But I also noticed that you
>boil the sugar together with the YNB; so I suspect that your plates are
>occassionaly not only mushy but also dark.

   If they are dark, then too long/hot might be the explanation. I
occasionally add sugar to my plates before autoclaving, and they usually
aren't any darker than adding after (20 minutes at 120C).

> It is always better to sterilize
>the carbon source separately and mix it to the rest immediately before
>pouring the plates (even for YEPD). Not only the plates look better this
>way, but also the yeasties grow MUCH better on them!

   I'm not sure about this. A yeast lab down the hall always adds sugar
before autoclaving. Sometimes they have YPD that is totally black. Of
course, I can't verify if they have problems with it...


This article is for entertainment purposes only. Any facts, opinions,
narratives or ideas contained herein are not necessarily true, and do
not necessarily represent the views of any particular person.  

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net