In article <33err9$20m at mserv1.dl.ac.uk>, <FATIMA at AIMP.UNA.AC.AT> wrote:
>is indeed hydrolysis of the agar. It makes a difference when you autoclave
>takes too long to get to the right temperature - (sorry: I meant YOUR auto-
>clave but I cannot return to correct it).
Get a real mailer :-)
> But I also noticed that you
>boil the sugar together with the YNB; so I suspect that your plates are
>occassionaly not only mushy but also dark.
If they are dark, then too long/hot might be the explanation. I
occasionally add sugar to my plates before autoclaving, and they usually
aren't any darker than adding after (20 minutes at 120C).
> It is always better to sterilize
>the carbon source separately and mix it to the rest immediately before
>pouring the plates (even for YEPD). Not only the plates look better this
>way, but also the yeasties grow MUCH better on them!
I'm not sure about this. A yeast lab down the hall always adds sugar
before autoclaving. Sometimes they have YPD that is totally black. Of
course, I can't verify if they have problems with it...
DanZ
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