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breaking yeast

Sandy Silverman sandy at icr.pt.cyanamid.COM
Fri Aug 19 13:17:08 EST 1994

In article <lopez.6.00119631 at medusa.bioc.aecom.yu.edu> lopez at medusa.bioc.aecom.yu.edu (Alfredo Lopez) writes:

>>>stuff deleted<<< 
  > through a French Press at 15,000psi and got only ~50% breakage or less.  I was
   > thinking of going to glass beads but am worried about inactivating the enzyme.
   > I would appreciate any suggestions.  Thanks

   > Susan Bromberg

>>   Unless lysozyme interferes with your assay, you can problably make speroplasts 
>>   before using the French press.  Even with a bead beater it will be hard to get 
>>   a 100% breakage.
>>	   See you on the dark side of the Moon.

  >> ------------------------------
  >> Alfredo Lopez-De-Leon         |
  >> lopez at medusa.bioc.aecom.yu.edu|
  >> A.E.C.O.M.                    |
  >> Biochemistry Department       |
  >> Bronx, NY                     |
  >> ------------------------------

Surely you mean Zymolyase and not lysozyme??

Sanford Silverman                      >Opinions expressed here are my own<
American Cyanamid  
silvermans at pt.cyanamid.com     			"Yeast is Best"

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