Sandy Silverman (sandy at icr.pt.cyanamid.COM) wrote:
: I would give glass beads a try since it is easy. Just make sure the tubes
: stay cold by vortexing a short time, put on ice, vortex, etc.
: --
In our lab, we break yeast in an osterizer blender with glass beads.
Take frozen cells, mix with 5 cell weights glass beads and one cell volume
buffer, and grind 5x 30sec. each with cooling on a salted ice bath between.
Maintain temperature below 4oC, (temp before each grinding session should
be below 1oC).
good luck.
--
jeff z-ler
ziehler at post.its.mcw.edu
waiting for the clu- phenotype to be a target for gene therapy.
