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breaking yeast

usmbcfm3 at IBMMAIL.COM usmbcfm3 at IBMMAIL.COM
Sat Aug 13 14:47:08 EST 1994

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From: Susan Bromberg_- usmbcfm3 at ibmmail.com
Research Department - Yeast Physiology and Fermentation
Subject: breaking yeast

 I am trying to break open an industrialaneuploid yeast strain for enzyme
 assays.  This strain seem to much prefer remaining intact.  I tried twice
 through a French Press at 15,000psi and got only ~50% breakage or less.  I was
 thinking of going to glass beads but am worried about inactivating the enzyme.
 I would appreciate any suggestions.  Thanks

 Susan Bromberg

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