>>A friend needs to find flanking sequences on yeast chromosome 2. He has a
>24 basepair sequence. Any suggestions?
I am not sure I get it. Does your friend happen to have 24 bp of sequence
that he is sure comes from Chr. II and wants to clone a hunk of genomic DNA
that includes this sequence? Has he tried searching the yeast sequence
database to see if it is already cloned? How about making a Chr II library
in pUC19 and then PCR out of it a conveniently sized fragment with the 24
bp sequence as one oligo and universal primer as the other?
jansma at sickkids.on.ca