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sequence of yc2

jansma at RESUNIX.RI.SICKKIDS.ON.CA jansma at RESUNIX.RI.SICKKIDS.ON.CA
Thu Aug 11 19:13:50 EST 1994


re:

>Hi,
>
>A friend needs to find flanking sequences on yeast chromosome 2.  He has a
>24 basepair sequence.  Any suggestions?
>
>Kath

I am not sure I get it.  Does your friend happen to have 24 bp of sequence
that he is sure comes from Chr. II and wants to clone a hunk of genomic DNA
that includes this sequence?  Has he tried searching the yeast sequence
database to see if it is already cloned?  How about making a Chr II library
in pUC19 and then PCR out of it a conveniently sized fragment with the 24
bp sequence as one oligo and universal primer as the other?

Dave 
jansma at sickkids.on.ca  




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