I was wondering whether anyone out there has tried using AGRIsol (Research
Products International) to isolate RNA from yeast?? We routinely use
acid-phenol freeze-thaw with OK results but we're working in Candida. We
have also tried Trizol reagent but the yield wasn't any different except
for a little more DNA contamination. RPI sells a couple of these RNA
isolation reagents for disrupting cells: RNA NOW, RNA NOW TC, and AGRIsol.
The latter is advertised as good for plant cells. But will it work on
yeasties?? The stuff is $85/100ml so just `trying it' is somewhat out of
our price range. For the Trizol I at least got a free sample. In that
case the company didn't know. I also tried it on Dictyostelium with no
Anyone with good experiences with this stuff??
Doug Rhoads || Dept. of Biological Sciences
drhoads at mercury.uark.edu || 601 Science Engineering
drhoads at uafsysb.uark.edu || University of Arkansas
501-575-3251 || Fayetteville, AR 72701