Dear Buddies,
On Sun, 31 Jul 1994, Kevin Morano wrote:
> The key to successful PCR amplification in our lab is Mg, Mg, Mg.
> A given set of primers and template will do absolutely nothing at say, 3
> mM Mg and will produce beautiful bands at 8-10 mM Mg.
My question is what if any effect does all that Mg++ have on your
polymerase of choice? My concern is that there could be a reduction in
fidelity leading to an increase in sequence changes in the PCR product.
Has anybody got some experience with sequencing PCR products produced at
such high Mg concentrations?
Mike Moser
Department of Biochemistry SJ-70
University of Washington moser at u.washington.edu
Seattle, WA 98195 Make peace my beast is yeast
