In Article <n1436870019.20295 at mail1.ciwemb.edu>, meluh at MAIL1.CIWEMB.EDU
("Pam Meluh") wrote:
>I'm interested in any VECTORS you may know of that are for expression and
>purification of proteins in yeast, particularly heterologous proteins. My
>colleague wants to express an Arabidopsis protein in yeast, as he has had
>difficulty doing so in E. coli. Ideally, he would like something with a
>polylinker that allows in-frame cloning to an EPITOPE TAG (e.g. FLAG, GST,
>His-6) that can be exploited for purification. The tag can be at the N- or
>C-terminus. If you know of anything, please let me know ASAP. Much thanks
>ahead of time.
I think that you will find what you need in:
Reisdorf et al (1993), Current Genetics 23, 181-183.
I hope this helps
Manuel G.
M. G. Claros ------------------------------- claros at biologie.ens.fr
Ecole Normale Superieure Genetique Moleculaire |/ \|
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