In message <9404202340.AA20822 at sequencer.wustl.edu> Mark Johnston writes:
> Hi buddies,
>> As part of our sequencing project here in St. Louis, we are
> planning a large-scale gene disruption project. In addition to disrupting
> genes, we would like also to construct promoter fusions to a reporter gene
> for measuring levels and regulation of gene expression. I am writing to
> ask for advice on reporters to use. The reporter must have the following
> properties:
>> 1. small size (< ~ 2kb), so it is PCRable;
>> 2. easy to assay (preferably with permealized cells);
>> 3. relatively sensitive assay, (so it can detect expression from
> weak promoters);
>> 4. no sequence homology to the yeast genome;
>> 5. (optional) provide a visual screen for activity;
>> 6. (optional) provide a selection for and/or against function (for
> selecting
> regulatory mutants).
>> If anyone knows of a good reporter that has these properties, I'd
> appreciate hearing about it. You can answer me directly, and I'll
> summarize the responses for the group. Thanks.
>> Sincerely,
>>> Mark Johnston
> Department of Genetics Box 8232
> Washington University Medical School
> 4566 Scott Ave.
> St. Louis, MO 63110
> FAX: 314-362-2985
> TEL: 314-362-2735
> E-mail: mj at sequencer.wustl.edu
Mark,
How about green fluorescent protein? Does anyone know if vectors are being
developed for using GFP as a yeast reporter?
Dave Eide
>>>
