To the Group,
I am looking for an inducible expression vector for S.
cerevisiae. It could be either 2 micron or CEN, preferably
with some sort of polylinker for cloning of the cDNA, and
without its own ATG. The vectors I used many years ago in
L. Guarente's lab are clumsy and uncharacterized. I'm
going to express some N. tabacum and Arabidopsis cDNAs
in yeast, mostly exploratory experiments. I apologize
if this question is asked too often.
Please respond to me and I will gladly summarize.
Thank you for your attention to this matter,
Brian Osborne
bosborne at nature.berkeley.edu
