> I have two questions I'd like some input on:
>> Can an enzyme be used to selectively remove uracil from YPD, so that yeast
> cells are growing under enriched conditions except for uracil? Our cells
> grow a day or two slower on synthetic medium as compared to YPD plates, so
> we were just curious if electrotransformed cells would survive easier on a
> YPD based medium in which uracil was enzymatically removed.
>> I'm just a plant physiologist who has a lot to learn about yeast! :-> Tama Fox
>>Hi Tama.
Why don't you grow the cells in liquid YEPD for one or two
generations after transformation, an then wash and plate on selective
medium (like you do with E.coli). Sometimes this helps.
Tomas Drgon
I'm just a yeast person who has a lot to learn about plant
physiology! :)
