In article <2obs4u$6o6 at masala.cc.uh.edu>, bchs1b at Elroy.UH.EDU (Michael
Benedik) wrote:
>> Can someone tell me the nature of the his3del200 mutation. Is this a deletion
> of his, how big etc.
>> thanks
>> ----------------------------------------------------------------------
> Michael Benedik INTERNET: Benedik at uh.edu> Dept. of Biochemical & Biophysical Sciences
> University of Houston BITNET: Benedik at uhou> -----------------------------------------------------------------------
The his3del200 allele consists of a 1 kb deletion that deletes the entire
coding region within the genomic HIS3 containing EcoRI/SalI fragment cloned
in the EcoRI and SalI sites of YRp14.
I got this information from Sikorski and Hieter, Genetics 122:19-27 (May
1989)
The original allele construction is described in Fasullo and Davis, MCB
8:4370-4380,1988 or Struhl and Hill, MCB 7:104-110, 1987.
Hope this helps!
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Scott J. Diede
The University of Chicago
Department of Molecular Genetics and Cell Biology
920 E 58th Street Box 56
Chicago IL 60637
TEL: (312) 702-2031
FAX: (312) 702-3172
died at midway.uchicago.edu
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