Colleagues,
Does anyone know of selective methods, as opposed to screening, to
isolate yeast transformants that have lost plasmids carrying auxotrophic
markers other than URA3? 5FOA selects for loss of URA3, but are methods
known that select for loss of TRP1, HIS3, or LEU2?
Alan Bakalinsky
Alan T. Bakalinsky, Ph.D.
Department of Food Science and Technology,
Wiegand Hall, Oregon State University
Corvallis, OR 97331-6602
Internet: bakalina at bcc.orst.edu
Phone: (503) 737-6510
FAX: (503) 737-1877
