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MET3 promoter

Randolph Hampton randy at mendel.berkeley.edu
Sun Oct 24 23:25:24 EST 1993


In article <2a77pc$f4a at news.u.washington.edu> Jim Sloan,
jsloan at carson.u.washington.edu writes:
>I found high levels of MET3 mRNA in yeast grown in glucose, but was 
>unable to detect any MET3 mRNA in yeast grown on a non-fermentable
carbon 
>source (ie ethanol and glycerol).  Therefore it would appear that the 
>MET3 promoter is regulated by carbon source.
That is very useful to know, Jim, so thanks! But two things: 1) so how
good is the repression of the Met3 promoter IN GLUCOSE when exogenous
Methionine is added? How much met does it take and how good is the shut
off? 2) Where does one obtain the Met3 promoter DNA for cloning and
Met-controlled expression?

R. Hampton
UC Berkeley MCB Dept
510-642-7070



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