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Why 2 % agar?

Fatima Cvrckova FATIMA at AT.AC.UNA.AIMP
Sun Oct 24 06:59:00 EST 1993

Dear Michael and others,

I think that the most likely reason why yeast methods suggest this concentration is 
that 2 % agar is less easy to "overcook" (i. e. partilally hydrolyze during 
autoclaving), especially in the slightly acidic yeast media. So, the real purpose is 
not making the yeast happier (yeast does not care - it grows as well in liquid) but 
to make you happier by ensuring that your plates are not too soft.


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