In article <2a6nef$lkl at agate.berkeley.edu>,
Randolph Hampton <randy at mendel.berkeley.edu> wrote:
>We would like any information, anecdotes, advice about using the
>methionine-suppressable promoter in yeast.
>UC Berkeley MCB Dept
I found high levels of MET3 mRNA in yeast grown in glucose, but was
unable to detect any MET3 mRNA in yeast grown on a non-fermentable carbon
source (ie ethanol and glycerol). Therefore it would appear that the
MET3 promoter is regulated by carbon source.
jsloan at u.washington.edu