Doug Lammer writes:
>I've just begun a yeast project in an Arabidopsis lab, and have a basic
>question about the most efficient way to get yeast libraries and plasmids.
>>My immediate needs are for a high copy number expression vector with URA3
>as the selectable marker, and for a genomic library suitable for a high
>copy suppressor screen also with URA as the selectable marker.
Point your gopher client at gopher.gdb.org and select:
12. Search Databases at Hopkins (Vectors, Promoters, NRL-3D, EST, OMIM./
--> 6. Culture Collections (ATCC)/
--> 1. ATCC - The American Type Culture Collection/
--> 7. Recombinant DNA Materials (Clones, Vectors, Libraries, Hosts, Oligo/
--> 2. Vectors <?>
and search for -
cerevisiae and URA3 and expression
If you're looking for high copy numbers - probably 2 micron plasmids, you
could search for:
cerevisiae and URA3 and expression and micron
to narrow it down a bit.
If you want inducible expression you could try:
cerevisiae and URA3 and expression and induc*
Best of luck,
danj at mail.gdb.org