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degenerate PCR

Dean Danner Lab djdlab at sun.com
Fri Oct 15 10:33:05 EST 1993


To anyone who is a PCR god or goddess,
	I am about to embark on a project involving PCR amplification of a not yet found yeast gene.  I am going to design the primers from human sequence.  Is it better to produce degenerate primers or primers using those codons preferred most by yeast?
	Does anyone know of a reference that has the preferred codon usage for yeast?

		Margaret M Lanterman
		Emory University



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