In regards to the question on screening yeast colonies by PCR we have used
a procedure for screening bacterial colonies and animal blood that should
work with just about anything.
1) Resuspend a small amount of cells from the colony (tip of a toothpick)
or 1-2 ul of whole animal blood in 100 ul sterile water.
2) place in boiling water bath for 10 minutes
3) spin 5' in the cold
4) use 2 in a 10ul or 5 ul in a 40ul PCR reaction
If you have problems with signal BioRad sells a purified chelex that you
can add some to the boil that pulls out some inhibitors
Doug Rhoads || Dept. of Biological Sciences
drhoads at mercury.uark.edu || 601 Science Engineering
drhoads at uafsysb.uark.edu || University of Arkansas
501-575-3251 || Fayetteville, AR 72701