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SNF1, SNF4 plasmids

Frank Li LI_N at MSGCG.WUSTL.EDU
Tue Dec 14 23:50:37 EST 1993


Hi, buddies,

    Interaction between yeast SNF1 and SNF4 proteins has been used in the two
hybrid system as a positive control. In this system, SNF1 is fused to the
GAL4 DNA binding domain and SNF4 is fused to the GAL4 transcription
activating domain.  Physical interaction between SNF1 and SNF4 will
reconstitute GAL4p activity. For some experiments I am going to do, I need
these two genes on the opposite plasmids such that SNF1 is fused to GAL4 AD
and SNF4 is fused to GAL4 BD. Any info or help is highly appreciated!

Have a great holiday! 

Frank
------------------------------
Frank Ning Li
Department of Genetics
P.O.Box 8232
Washington University
4566 Scott Avenue
St. Louis, MO 63110

Tel: (314)-362-5799
Fax: (314)-362-7855
Email: LI_N at msgcg.wustl.edu
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