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LacZ fusions

Manuel G. CLAROS claros at biologie.ens.fr
Wed Dec 8 08:14:23 EST 1993

In Article <9312032218.AA05363 at mx.nsi.nasa.gov>, FOGEL at biovx1.span.nasa.gov
>Fellow yetties,
>I'm using a plasmid with a Gal1-lacZ fusion and finding that there is
>considerable variation in the blue color of the yeast colonies (even though
>I've tried plating with different concentrations of X-Gal).  Would anyone
>happen to have another idea as to why this might be happening?  Any good
>references on color variation in these fusions would be quite helpful.
>Gary Fogel
>U.C.L.A. Dept of Biology
>405 Hilgard Av.
>Los Angeles, CA 90024-1606
>(310) 206-7809

Have you tried to see if you are losting your plasmid? sometimes in b-gal plates
it is usual and you have not reproductible results

!  Manuel G. CLAROS                 !
!  Ecole Normale Superieure - CNRS  !
!  Lab. Genetique Moleculaire       !
!  46 rue d'Ulm                     !
!  75005 Paris (France)             !
!  E-mail: claros at biologie.ens.fr   !
!  Fax:    331-44 32 39 41          !

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