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LacZ fusions

FOGEL at biovx1.span.nasa.gov FOGEL at biovx1.span.nasa.gov
Fri Dec 3 17:26:48 EST 1993


Fellow yetties,

I'm using a plasmid with a Gal1-lacZ fusion and finding that there is
considerable variation in the blue color of the yeast colonies (even though
I've tried plating with different concentrations of X-Gal).  Would anyone
happen to have another idea as to why this might be happening?  Any good
references on color variation in these fusions would be quite helpful.

Thanks,

Gary Fogel
U.C.L.A. Dept of Biology
405 Hilgard Av.
Los Angeles, CA 90024-1606
(310) 206-7809



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