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counterselection scheme for LEU2

Morris F. Manolson mm6y+ at andrew.cmu.edu
Thu Apr 22 12:41:51 EST 1993


> 
>    Is there any counterselectionn scheme for LEU2. Something like
>FOA used to select against the URA3 gene.

Not that I know of but if you can't use URA3 
how about using LYS2 and then counterselecting with 
DL-alpha-aminoadipic acid?  Morrie



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