In article <C5ItxD.8Iw at liverpool.ac.uk> beynonrj at liverpool.ac.uk (Dr. R.J. Beynon) writes:
>OK. I am a biochemist who works with a large, furry multicellular 'yeast'
>-otherwise known as a mouse. If I want to work on a real yeast, it is a
>major shift in thinking/methods/strategies. Question: what is the best
>book that might let me start to explore the yeast system - specifically for
>protein degradation studies, but that may not be relevant - from the
>idiot's guide to the sophisticated manuals. Do they exist?
>>(I also appreciate that there is no substitute for talking to or working
>with a real expert!)
>>Suggestions please-
>>Rob Beynon, Biochemistry, Liverpool
As for a practical manual for methods and strategies, our lab relies
on the fairly recent "Guide to Yeast Genetics and Molecular Biology:
Methods in Enzymology" edited by Christine Guthrie and Gerald Fink,
published by Academic Press, ISBN 0-12-182095-5 (paperback). This
book has a lot of very useful molecular genetic and biochemical
techniques coverig subjects that will be useful to any novice or
seasoned yeast biologist. Mark Hochstrasser here at Univ of Chicago
works on the ubiquitin protein degradation system in yeast, and is
recently from Alex Varshavsky's lab at MIT. His lab number is
(312)-702-2117 and the FAX number for his department is 702-0439. His
e-mail address is hoc1 at midway.uchicago.edu, but I don't know if he
reads e-mail.
Bob Litt
rjl4 at midway.uchicago.edu
University of Chicago
