Salamander info wanted

IU Axolotl Colony axolotl at indiana.edu
Fri Sep 24 09:24:54 EST 2004

Hello all,
We recently received the email below and thought we'd pass it along to 
increase the chances that the query will be seen by a wider audience, 
hopefully to increase the amount of info we can get for the sender.

Anyone with input please respond to the IU Axolotl Colony, or to the 
sender directly.  Thanks in advance for your help.
IU Axolotl Colony staff

> From: Ben Ridenhour <snake8mynewt at yahoo.com>
> Date: September 8, 2004 5:14:40 PM EST
> Subject: Salamander info
> Hello,
> I'm considering the possibility of creating an clonal line of A. 
> tigrinum for research purposes using androgenesis.  I was wondering if 
> someone could provide some information about such a project.  Namely, 
> I am looking for a protocol for A. mexicanum that I could use in A. 
> tigrinum.  Gillespie and Armstrong (1980) published a paper on 
> creating adrogentic diploids in axolotls-- has there been more recent 
> research on this?

> We were also considering using axolotl eggs (to be irradiated) and 
> fertilizing them with tigrinum sperm to maintain a line.  Do know if 
> this would be possible?  Part of this would require cryogenically 
> preserving salamander sperm -- is that possible?  Any
> help/guidance in this matter would be wonderful (I'm a strict novice 
> at this).  Thanks for you time--
> Ben Ridenhour

> =====
> Benjamin Ridenhour
> School of Biological Sciences
> Washington State University
> P.O. Box 644236
> Pullman, WA 99164-4236
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