I am looking for any information anyone can give me about attempts,
successful or unsuccessful, to immortalize cells of urodeles
or other amphibians. I am specifically interested in using the calcium
phosphate precipitation method to transfect primary cultures of small
and large intestinal epithelial cells of axolotls with the SV40 large T
antigen vector. To work, the retinal blastoma (rb) gene equivalent or
the P53 gene equivalent must be present. Do urodeles have the rb or
P53 genes? Related to this, have there been attempts at making
transgenic urodeles? Thanks in advance for your help with these questions
or any comments you might have.
jfwhite at physio.emory.edu
John F. White
Dept. of Physiology Tel # 404-727-7418
Emory University FAX # 404-727-2648
Atlanta, GA 30322 email jfwhite at physio.emory.edu
