cryostat sectioning of axolotl embryos

Linda Barlow lbarlow at ucsd.edu
Thu Jan 18 13:23:06 EST 1996

I am having trouble cutting NICE crytostat sections of axolotl embryos
(stage 14 - 25).  The block itself cuts well, i.e. the sections are not
torn, but the tissue is horribly shattered.  Does anyone have any special
procedures they use to get good, intact frozen sections?? (By the way, my
method works fine on older embryos and early larvae).

Thanks for any advice...

Linda Barlow

Linda A. Barlow                         Phone: 619-534-5618
Dept. of Neurosciences 0201             FAX:   619-534-5622
University of California, San Diego
La Jolla, CA  92093-0201

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