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HELP with Minimum Inhibitory Concentration (MIC) calculations

Nathaniel Sopelario nate at netspace.net.au
Wed Mar 27 06:07:58 EST 1996


Mark Fuller wrote:
> 
> I have just conducted some experiments to determine the minimum
> inhibitory concentration (MIC) of a chemical and a bunch of different
> bacteria.  I have data covering the initial compound concentrations, the
> optical density (OD) over time of the cultures and the final compound
> and metabolite concentrations.
> 
> 1) What should be graphed versus what?  Initial concentration vs. OD
> gives a good trend, but is there any model/equation I can use to figure
> out the MIC?  I can eyeball it, but I'd much rather do it
> mathematically.
> 
> 2) What's the general feeling of reporting LC-50, EC-50 along with MIC
> values?
> 
> Any input or hints on specific references would be much appreciated.
> 
> -Mark Fuller, Ph.D.

Mark,
   In Excel 5, there's a tool to have a mathematical representation of 
the graph created by the variables chosen. You can play around with it 
to determine an equation. 

Nathaniel Sopelario



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