[Protein-analysis] Help me! AIR BUBBLES trapped in HPLC column...

Joana Arroz via proteins%40net.bio.net (by joanaarroz from isa.utl.pt)
Wed Jan 13 04:46:17 EST 2016


it´s not good to dry your column. to take the air off you need to increase
the pressure inside the column, so close the exit for a few seconds,
respect the maximum pressure of the column.

after this, you need to check your column, to see if it working properly.

best regards,
Joana Arroz

2016-01-10 23:32 GMT+00:00 Bilal Majed <majedbilal from gmail.com>:

> Hello
> After I had completed my analysis, I switched to sequence flush overnight
> for two hours. However I made an error in the settings and realised in the
> next morning the method was still running and I ran out of wash phase. So
> air was being pumped into the system and the column.
> Please can you help me correct this mistake.
> Hplc system- Agilent 1220 Infinity LC System
> Column- Inertsil ODS 3V
> Reverse phase system
> Thank you
> Bilal Majed
> Sent from my iPhone
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