[Protein-analysis] Help me! AIR BUBBLES trapped in HPLC column...

Bilal Majed via proteins%40net.bio.net (by majedbilal from gmail.com)
Sun Jan 10 18:32:00 EST 2016

After I had completed my analysis, I switched to sequence flush overnight for two hours. However I made an error in the settings and realised in the next morning the method was still running and I ran out of wash phase. So air was being pumped into the system and the column. 

Please can you help me correct this mistake.

Hplc system- Agilent 1220 Infinity LC System
Column- Inertsil ODS 3V
Reverse phase system

Thank you

Bilal Majed

Sent from my iPhone

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