[Protein-analysis] Citrate Buffer - Carboxylation of proteins and peptides ?

[Straube,Werner]

Dear all,

I intend to use citrate buffer at 100 mM for some experiments, but then I stumbled over a notice from Dionex, that citrate buffer can modify proteins and peptides.

However, when searching for references I was not able to find information about it. Is this really a major artefact in citrate buffer ?

Does anyone have some information or references about it ?

Thanks,

Werner