In article <mailman.35.1289798685.15153.proteins from net.bio.net>,
r.zhang from auckland.ac.nz says...
>> I would like to know if it is possible to do western blotting experiment
> using formalin fixed frozen tissues of rat brain, thanks for that Rong
Difficult, as formalin cross-links proteins. The more common method is
to rehydrate sections and elute proteins with hot RIPA-buffer containing
detergents. The proteins are reduced, carboxymethylated and protease
digested. The resulting peptides are separated by capillary
electrophoresis and identified by MS/MS. For a review see Nirmalan et
al.: Mol. Biosyst. 4 (2008) 712-20